DNA methylation alterations in prostate cancer patient derived xenograft models revealed by whole genome bisulfite sequencing. DNA methylation alterations in prostate cancer patient derived xenograft models revealed by whole genome bisulfite sequencing

DNA methylation alterations are a universal feature of cancer. In prostate cancer, site specific DNA methylation changes have been suggested as driver in disease initial and progression. Here we provide a comprehensive assessment of DNA methylation changes in prostate cancer patient derived xenograft (PDX) models. We delineate patterns of both site specific and global methylation changes and nominate novel candidates for biomarker development. Overall design: Whole genome bisulfite sequencing of PDX samples

DNA甲基化改变是癌症的普遍特征。在前列腺癌中，位点特异性DNA甲基化变化已被提示为疾病起始与进展的驱动因素。本研究针对前列腺癌患者来源异种移植（Patient Derived Xenograft, PDX）模型中的DNA甲基化改变开展了全面评估，系统阐明了位点特异性与全局甲基化变化的模式，并筛选出可用于生物标志物开发的新型候选靶点。整体实验设计：对PDX样本进行全基因组亚硫酸氢盐测序