Stability of NTS1 in the absence of agonist.

[3H]NT binding to the NTS1 fusion protein was recorded over time and half-lives were calculated. The amount of functional NTS1 remaining after 6 hrs (NiE and diluted NiE) and after 24 hrs (SN) was estimated from one phase exponential decay fits. Abbreviations: SN, supernatant after ultracentrifugation; NiE, Ni-NTA column eluate.

[3H]NT与NTS1融合蛋白（NTS1 fusion protein）的结合反应随时间进程被动态记录，并计算得到其半衰期。通过单指数衰减拟合，估算出6小时后（NiE及其稀释液）以及24小时后（SN）仍具有功能活性的NTS1的含量。缩写注释：SN指超速离心后的上清液（supernatant after ultracentrifugation）；NiE指Ni-NTA柱洗脱液（Ni-NTA column eluate）。