CIL:35374, Homo sapiens, cevical carcinoma. In Cell Image Library

HeLa cells were metaphase-arrested by a 3hr treatment with colcemid, the cells mechanically broken by passage through a 22-gauge needle and exposed to hypotonic 75 mM KCl, treated to remove histones, spread on a water surface, fixed with formaldehyde, picked up on formvar coated grid, critical point dried, and imaged with the Wisconsin HVEM at 1 MeV. See Ris (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246; Ris (1981) Stereoscopic microscopy of chromosomes. Meth Cell Biol 22:77-96.

海拉（HeLa）细胞经秋水仙酰胺（colcemid）处理3小时后被阻滞于有丝分裂中期；随后通过22号针头对细胞进行机械裂解，将细胞置于75mM低渗氯化钾溶液中处理，经去组蛋白操作后将样本铺展于水相表面，以甲醛固定后捞取至福姆瓦（Formvar）包被载网，经临界点干燥后，使用威斯康星高压电子显微镜（HVEM）以1MeV加速电压完成成像。详见以下两篇文献：Ris（1978）《用于电子显微镜的染色质与染色体制备方法》，《细胞生物学方法（Meth Cell Biol）》18:229-246；Ris（1981）《染色体体视显微技术》，《细胞生物学方法（Meth Cell Biol）》22:77-96。