Triplication of the Ifnr locus exacerbates craniofacial anomalies in a mouse model of Down syndrome.

Micro-computed tomography (mCT) reconstructions of skulls from the Dp16 mouse model of Down syndrome, Dp16 corrected for <em>Ifnr </em>dosage (Dp16^2xIfnrs), and wildtype controls (WT). Mice were decapitated at 7-8 weeks of age then whole heads imaged using a SkyScan 1275. Scanning was performed at 17.6-micron resolution using the following parameters: 55 kV, 180 mA, 0.5 mm Al filter; 0.3˚ rotation step over 180˚, and 3-frame averaging. All raw scan data were reconstructed to multiplanar slice data using NRecon v1.7.4.6 (Bruker, Belgium). Reconstructed data were then rendered in 3D with consistent thresholding parameters using Drishti v2.6.5 for gross visual assessment of the craniofacial skeleton. Representative rendered images were captured and processed using Photoshop 24.2.0 (Adobe Creative Cloud). <br>

本数据集包含针对唐氏综合征Dp16小鼠模型、Ifnr剂量校正型Dp16小鼠（Dp16^2xIfnrs）及野生型对照（WT）的颅骨所采集的显微计算机断层扫描（micro-computed tomography，mCT）重建数据。实验小鼠于7~8周龄时断头取头，随后使用SkyScan 1275扫描仪对完整头部进行成像。扫描参数设置如下：分辨率17.6微米，管电压55kV，管电流180mA，配备0.5mm铝滤片；扫描覆盖180°范围，旋转步距0.3°，采用3帧平均成像模式。所有原始扫描数据均使用比利时布鲁克（Bruker）公司的NRecon v1.7.4.6软件重建为多平面切片数据。随后以统一的阈值参数，使用Drishti v2.6.5软件对重建数据进行三维渲染，用于颅面骨骼的大体视觉评估。代表性渲染图像通过Adobe Creative Cloud旗下的Photoshop 24.2.0软件采集并处理。