HDAC1 occupation and chromatin accessibility in Adv-Hey2-transduced and siRNA-Hey2 knockdown NRVMs

Given that class I histone deacetylases are involved in cardiac homeostasis, we postulated that Hey2 recruits Hdac1, an abundantly expressed Hdac in adult hearts, to mediate the transcriptional repression of energy gene expression. To test the idea, we performed Hdac1 CUT&Tag-seq and ATAC-seq in Adv-Hey2-transduced and siRNA-Hey2 knockdown NRVMs.

鉴于I类组蛋白去乙酰化酶（class I histone deacetylases）参与心脏稳态调控，我们提出假说：Hey2可募集成年心脏中高表达的组蛋白去乙酰化酶1（Hdac1），以介导能量基因表达的转录抑制。为验证该假说，我们对腺病毒介导Hey2转导以及经Hey2小干扰RNA敲低的新生大鼠心室肌细胞（NRVMs）进行了Hdac1 CUT&Tag测序与转座酶可及性测序（ATAC-seq）。