Microarray analysis of c-Jun/JunB knockout mice treated with dithranol

Ears of c-Jun/JunB knockout mice were topically treated with dithranol Mice carrying floxed alleles for the JunB and c-Jun locus and expressing the K5-CreERT transgene received consecutive i.p. injections of tamoxifen for a period of 5 days, leading to deletion of both genes in the epidermis by inducible Cre-recombinase activity. One week after the last injection, psoriasis-like lesions on the ears were treated daily with vehicle (Vaseline) or dithranol in series of increasing concentrations.

对c-Jun/JunB双基因敲除小鼠的耳部实施蒽林（dithranol）局部处理。携带JunB与c-Jun基因座loxP侧翼等位基因（floxed alleles）、且表达K5-CreERT转基因（K5-CreERT transgene）的小鼠，连续5天接受腹腔内（i.p.）注射他莫昔芬，通过诱导型Cre重组酶活性实现表皮内双基因的敲除。末次注射一周后，每日以赋形剂（凡士林，Vaseline）或梯度浓度蒽林处理该小鼠耳部的银屑病样皮损。