16S rRNA sequencing of C57 mouse fecal samples

The project was divided into four groups(n=5 per group): the low-fat diet group(LC), the low-fat diet plus periodontitis group(LP), the high-fat diet group(HC), and the high-fat diet plus periodontitis model group(HP). the low fat diet group made up 10% of their total caloric intake, while the high-fat diet group comprised 60% of their total caloric intake.Liver steatosis was observed in mice following 12 weeks of HFD feeding. Then, mice fed low-fat and high-fat diets were randomly assigned to ligated and unligated groups. The ligated groups (LP and HP) underwent periodontitis induction via ligation of the maxillary second molars using 7.0 suture. LC_J69, LC_J70, LC_J77, LC_J80, LC_J81; HC_J32, HC_J34, HC_J35, HC_J36, HC_J37; LP_B51, LP_B53, LP_B54, LP_B55, LP_B56; HP_A79, HP_J8, HP_J9, HP_J10, HP_J58.

本实验分为4组，每组n=5：低脂饮食组（LC）、低脂饮食联合牙周炎组（LP）、高脂饮食组（HC）及高脂饮食联合牙周炎模型组（HP）。其中低脂饮食提供的热量占小鼠总摄入热量的10%，高脂饮食提供的热量占小鼠总摄入热量的60%。高脂饮食（High Fat Diet, HFD）喂养12周后，小鼠出现肝脏脂肪变性。随后，接受低脂饮食与高脂饮食喂养的小鼠被随机分为结扎组与非结扎组。结扎组（LP与HP）通过以7.0号缝线结扎上颌第二磨牙的方式诱导牙周炎。各组小鼠编号如下：LC组：LC_J69、LC_J70、LC_J77、LC_J80、LC_J81；HC组：HC_J32、HC_J34、HC_J35、HC_J36、HC_J37；LP组：LP_B51、LP_B53、LP_B54、LP_B55、LP_B56；HP组：HP_A79、HP_J8、HP_J9、HP_J10、HP_J58。