Measuring the influence of RNA binding proteins on A-to-I RNA editing in the Drosophila brain

A-to-I RNA editing levels differ across tissues and cell types, but regulators of the editing process are largely unknown. We used RNA-seq on whole fly brains with different RNA binding proteins knocked down to test for A-to-I RNA editing level differences between controls and knockdowns. To screen for editing regulators in the Drosophila brain, we crossed a pan-neuronal Gal4 driver, C155-Gal4, to different UAS-shRNA lines targeting individual RNA binding proteins, extracted RNA and made RNA-seq libraries. We sequenced four total replicates of shGFP controls and two replicates of all RNA binding protein knockdowns.

A-to-I RNA编辑（A-to-I RNA editing）的水平在不同组织与细胞类型间存在差异，但该编辑过程的调控因子大多尚未明确。我们对敲低了不同RNA结合蛋白（RNA binding protein）的完整果蝇全脑组织开展RNA测序（RNA-seq），以检测对照组与敲低组之间的A-to-I RNA编辑水平差异。为筛选果蝇（Drosophila）大脑中的编辑调控因子，我们将泛神经元Gal4驱动子（Gal4 driver）C155-Gal4与靶向单个RNA结合蛋白的不同UAS-shRNA株系进行杂交，随后提取RNA并构建RNA测序文库。我们共对4个shGFP对照组的生物学重复样本，以及所有RNA结合蛋白敲低组的2个生物学重复样本进行了测序。