Metformin Regulates Ferroptosis in Skin Cutaneous Melanoma

To explore the effects of metformin on the proliferation and ferroptosis of skin cutaneous melanoma (SKCM) and its potential molecular mechanisms, providing a new theoretical basis and strategy for the treatment of cutaneous melanoma. RNA-seq sequencing and related analyses were used to screen differentially expressed genes and explore their involved biological functions and signaling pathways. RNA-seq analysis found 2068 differentially expressed genes, of which 897 were up-regulated and 1171 were down-regulated. The related pathways such as iron metabolism disorders and ferroptosis were activated. Overall design: Exploring the molecular mechanism of metformin in regulating A375 melanoma cells in the skin

本研究旨在探究二甲双胍对皮肤黑色素瘤（skin cutaneous melanoma, SKCM）细胞增殖与铁死亡的影响及其潜在分子机制，为皮肤黑色素瘤的临床治疗提供全新理论依据与干预策略。本研究采用RNA测序（RNA-seq）及相关分析方法筛选差异表达基因，并解析其参与的生物学功能与信号通路。经RNA-seq分析，共筛选得到2068个差异表达基因，其中897个基因上调、1171个基因下调；铁代谢紊乱、铁死亡等相关通路被激活。整体实验设计：探究二甲双胍调控皮肤A375黑色素瘤细胞的分子机制。