Effects of Inhibition of CDK8/19 Mediator Kinase by Senexin B in HCT116 cells treated with or without TNF-alpha

We have investigated the effects of CDK8/19 inhibitor Senexin B on NFκB-mediated transcription, induced by a canonical NFκB activator, TNFα. HCT116 cells were pre-treated with or without 1 μM Senexin B for 1 hour, followed by 2 hour treatment with 10 ng/ml TNFα. Each treatment condition was done in biological triplicate.

本研究探究了细胞周期蛋白依赖性激酶8/19（CDK8/19）抑制剂Senexin B对经典核因子κB（NFκB）激活剂肿瘤坏死因子α（TNFα）诱导的NFκB介导转录的影响。实验中，将HCT116细胞以1 μM Senexin B或对照溶剂预处理1小时，随后以10 ng/ml的TNFα处理2小时。所有处理条件均设置3次生物学重复。