RAW Data-Virulence247762554.xlsx

担子菌 Sporisorium scitamineum 引起甘蔗黑穗病，导致甘蔗的质量和产量严重受损。 多胺影响细胞内氧化还原平衡，这对 致病性发展 的S. scitamineum，包括有配、双核菌丝生长,和宿主入侵。 中鉴定了一种多胺转运蛋白 SsTPO1S. scitamineum。SsTPO1导致细胞内腐胺、亚精和精向相反方向破坏。基于 SsTPO1 的多胺稳态有助于有配后双核菌丝生长和宿主入侵，可能是通过影响 cAMP-PKA 信号通路和协同调节氧化还原稳态。环境 pH 值影响 SsTPO1 介导的多胺运输，因此 S. scitamineum 菌丝的生长有利于中性至碱性环境，而不是酸性条件。基于 SsTPO1 的多胺转运也参与抗应激和自噬诱导。总之，我们的工作揭示了 SsTPO1 促进多胺转运，以调节 和真菌致病性 的S. scitamineum。

Sporisorium scitamineum, a basidiomycete fungus, causes sugarcane smut, which severely impairs the quality and yield of sugarcane. Polyamines affect intracellular redox homeostasis, which is critical for the pathogenic development of S. scitamineum, including mating, dikaryotic hyphal growth, and host invasion. A polyamine transporter, SsTPO1, was identified in S. scitamineum. SsTPO1 disrupts the intracellular transport of putrescine, spermidine and spermine in opposite directions. SsTPO1-mediated polyamine homeostasis contributes to post-mating dikaryotic hyphal growth and host invasion, potentially by regulating the cAMP-PKA signaling pathway and coordinately modulating redox homeostasis. Environmental pH affects SsTPO1-mediated polyamine transport, and thus the growth of S. scitamineum hyphae is favored in neutral to alkaline environments rather than acidic conditions. SsTPO1-mediated polyamine transport is also involved in stress resistance and autophagy induction. In conclusion, our work reveals that SsTPO1 promotes polyamine transport to regulate the fungal pathogenicity of S. scitamineum.