RNA-seq analysis of MEF cells with stably overexpression of IDH2 mutation and MEF cells treated with 2-HG

Isocitrate dehydrogenase 2 (IDH2) mutations and its key effector 2-hydroxyglutarate (2-HG) have been reported to promote oncogenesis in various human cancers. To elucidate molecular mechanism(s) associated with IDH2 mutations, we established mouse embryonic fibroblasts (MEF) cells stably expressing cancer-associated IDH2R172S mutation and treated MEF cells with or without 2-HG. The expression of genes in these cells were analyzed by RNA-seq. Overall design: Expression profiles of MEF cells stably expressing mutant IDH2 or mock vector, and MEF cells treated with 300 µM of 2-HG or DMSO for 24 h

异柠檬酸脱氢酶2（Isocitrate dehydrogenase 2, IDH2）突变及其关键效应物2-羟基戊二酸（2-hydroxyglutarate, 2-HG）已被报道可在多种人类癌症中促进肿瘤发生。为阐明与IDH2突变相关的分子机制，本研究构建了稳定表达癌症相关性IDH2R172S突变的小鼠胚胎成纤维细胞（mouse embryonic fibroblasts, MEF），并对该细胞分别施加2-HG处理与空白对照处理。采用RNA测序（RNA-seq）技术分析了这些细胞中的基因表达情况。
实验设计概述：本研究分析的表达谱来自以下四类样本：稳定表达突变型IDH2或空载体（mock vector）的MEF细胞，以及经300 μM 2-HG或二甲基亚砜（dimethyl sulfoxide, DMSO）处理24小时的MEF细胞。