Expression data from endothelial cells sorted from breast cancer cells MDA-MB231 as compared with normal endothelial cells. Homo sapiens

We studied the crosstalk between tumor and endothelial cells to explore the role of tumor microenvironment on cancer growth and progression. As part of our investigation, we showed that contact-dependent interaction of endothelial cells with breast tumor cells triggered the differential expression of a large number of genes in endothelium. Overall design: Endothelial and MDA-MB231 cells were co-cultured together under serum- cytokine-free environment for 5 days followed by sorting endothelial cells and extracting RNA for microarray analysis. All conditions were made in triplicate. Endothelial cells were obtained using the methods described in Seandel M. & Butler J.M., 2008. WCMC-Q Genomics Core

我们针对肿瘤与内皮细胞间的细胞串扰（crosstalk）展开研究，旨在探究肿瘤微环境（tumor microenvironment）对癌症生长与进展的调控作用。本研究证实，内皮细胞与乳腺肿瘤细胞的接触依赖性相互作用，可诱导内皮细胞内大量基因发生差异表达。实验整体设计：将内皮细胞与MDA-MB231细胞置于无血清无细胞因子环境中共培养5天，随后分选出内皮细胞并提取核糖核酸（RNA），用于基因微阵列分析（microarray analysis）。所有实验条件均设置三次生物学重复。内皮细胞的获取方法参照Seandel M.与Butler J.M.于2008年发表的研究方案，实验依托卡塔尔威尔康奈尔医学院基因组核心实验室（WCMC-Q Genomics Core）完成。