Transcriptome differences in FCRL5 positive and negative Influenza-specific memory B cells

We examined the transcriptional differences between circulating B cell subsets post-vaccination. Using FCRL5 as a marker of Tbet expressing B cells, we sorted FCRL5+ (Tbet+) and FCRL5- (Tbet-) memory B cells, naïve B cells, and antibody secreting plasmablasts at day 7 and day 14 post-vaccination. Additionally, we used H1 B cell tetramers to track cells responding to the influenza vaccine. These data provide insights into the transcriptional programming of distinct B cell subsets that respond to influenza vaccination. Overall design: RNA-seq on 4 B cell subsets from patients 7 or 14 days post influenza vaccination.

本研究探究了疫苗接种后循环B细胞亚群的转录差异。以FCRL5作为表达Tbet的B细胞标记物，我们在流感疫苗接种后第7天和第14天，分选出FCRL5阳性（Tbet阳性）与FCRL5阴性（Tbet阴性）记忆B细胞、初始B细胞，以及抗体分泌性浆母细胞。此外，本研究使用H1型B细胞四聚体追踪对流感疫苗产生应答的细胞。本数据集可为解析流感疫苗应答相关的不同B细胞亚群的转录编程提供参考依据。整体实验设计：对流感疫苗接种后第7天或第14天的患者样本中的4种B细胞亚群进行RNA测序（RNA-seq）。