Genome-wide DNA methylation analysis of non-cancerous urothelium obtained from patients with urothelial carcinomas and corresponding cancerous tissue

The aim of this study was to establish criteria for carcinogenetic risk estimation in the urothelium. Forty-six samples of non-cancerous urothelium showing no remarkable histological findings obtained from patients with urothelial carcinomas (N), and 46 samples of the corresponding cancerous tissue (T), i.e. 92 tissue samples in total, were analyzed. Genome-wide DNA methylation analysis was performed using the Infinium HumanMethylation 450K BeadChip. Bisulphite converted DNA from the 92 samples were hybridised to the Illumina Infinium HumanMethylation 450K BeadChip.

本研究旨在建立尿路上皮致癌风险评估的判定标准。本研究共纳入92份组织样本进行分析：其中46份为来自尿路上皮癌患者、无显著组织学异常的非癌性尿路上皮样本（标记为N组），剩余46份为对应的癌组织样本（标记为T组）。本研究采用Infinium HumanMethylation 450K BeadChip开展全基因组DNA甲基化分析。将92份样本的亚硫酸氢盐转化DNA与Illumina Infinium HumanMethylation 450K BeadChip进行杂交。