Gene expression analysis in lungs of Mtb infected Fth+/+ and Fth-/- mice by employing RNA-Seq. Gene expression analysis in lungs of Mtb infected Fth+/+ and Fth-/- mice by employing RNA-Seq

Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study is to compare gene expression (RNA-seq) profile between Mtb infected Fth+/+ and Fth-/- mice at 9 weeks post Mtb infection. Gene expression analysis demonstrated an essential role for FtH in mitochondrial function and maintenance of central intermediary metabolism in vivo Overall design: Total RNA was isolated from lungs of Mtb infected Fth+/+ and Fth-/- mice at 9 weeks post infection

下一代测序（Next-generation sequencing, NGS）已彻底革新了细胞通路的系统级分析。本研究旨在对比结核分枝杆菌（Mycobacterium tuberculosis, Mtb）感染后9周时，Fth+/+与Fth-/-小鼠的基因表达（RNA测序, RNA-seq）谱差异。基因表达分析揭示了铁蛋白重链（Ferritin heavy chain, FtH）在体内线粒体功能维持与中枢中间代谢稳态维持中的关键作用。整体实验设计：于结核分枝杆菌感染后9周，从Fth+/+与Fth-/-小鼠的肺部组织中提取总RNA。