Single cell qRT-PCR (scPCR) for rat hepatocytes and biliary epithelial cell (BEC)-enriched non-parenchymal cells (NPCs).. Single cell qRT-PCR (scPCR) for rat hepatocytes and biliary epithelial cell (BEC)-enriched non-parenchymal cells (NPCs).

Using FACS-sorted adult rat hepatocytes with distinct DNA contents (2c and 4c), we compared the expression levels of a 47 gene panel. As non-hepatic control samples, BEC-enriched NPCs were also used for the same scPCR experiment. Overall design: 92 2c hepatocytes and 245 4c hepatocytes were prpared by C1 single cell capture system.

本研究采用经荧光激活细胞分选（Fluorescence-Activated Cell Sorting，FACS）分离得到的、具有不同DNA含量（2c与4c）的成年大鼠肝细胞，对47基因表达面板的表达水平开展了比较分析。作为非肝源性对照样本，富集胆管上皮细胞的肝非实质细胞（BEC-enriched NPCs）同样被应用于本次单细胞PCR（single-cell PCR，scPCR）实验。实验整体设计：通过C1单细胞捕获系统（C1 single cell capture system）制备得到92个2c肝细胞与245个4c肝细胞。