Soil microbiome of a site contaminated with heavy metals. Soil microbiome of a site contaminated with heavy metals

This study used eDNA metabarcoding to characterize the soil microbiome at five depths in a brownfield site contaminated with heavy metals, petroleum hydrocarbons, and PAHs. The markers targeted were: 16S rRNA, 18S rRNA, and fungal ITS markers. The raw data generated were amplicon fastq sequences.

本研究采用环境DNA（environmental DNA） metabarcoding技术，对一处受重金属、石油烃与多环芳烃（polycyclic aromatic hydrocarbons, PAHs）污染的棕地场地内五个梯度深度的土壤微生物组开展表征分析。本研究靶向的分子标记包括16S核糖体RNA（16S rRNA）、18S核糖体RNA（18S rRNA）以及真菌内转录间隔区（internal transcribed spacer, ITS）标记。所获得的原始数据为扩增子fastq序列。