Metagenome from microbial community of plankton and sediment

In this work, we have characterized the microbial diversity in mesopelagic and bathypelagic zones of the water column (500, 1000 m and 1250 m depth) and bottom sediment from the Marmara Sea by amplifying, cloning and sequencing archaeal, bacterial and eukaryotic small-subunit rRNA (SSU rRNA) genes and by direct pyrosequencing of environmental DNA from 1000 m-deep plankton and sediment (approximately 35 Mbp, respectively). The plankton sample was recovered from the water column in the Central Basin of the Marmara Sea (40° 50.3'N 28°1.4' E) at 1000m depth. The sediment sample was recovered from the Central Basin of the Marmara Sea at 1,300 m depth (40° 46.8'N and 29°6.1' E). A comparative metagenomics analysis of these data with that of previous studies of deep-sea and surface plankton, subsurface deep-sea sediment, whale carcasses and soil reveals interesting trends. HM103388-HM103902 are ribosomal RNA sequences associated with this study.

本研究针对马尔马拉海水体中层带与半深海带（水深500米、1000米及1250米）及海底沉积物的微生物多样性展开表征：通过扩增、克隆并测序古菌、细菌与真核生物的小亚基核糖体RNA（small-subunit rRNA, SSU rRNA）基因，同时对1000米水深的浮游生物与沉积物的环境DNA进行直接焦磷酸测序（单样本数据量约35 Mbp）。本次研究的浮游生物样本采集自马尔马拉海中央盆地水深1000米处的水柱，采样坐标为北纬40°50.3'、东经28°1.4'；沉积物样本则采集自该海域中央盆地水深1300米处，采样坐标为北纬40°46.8'、东经29°6.1'。将本研究数据与此前深海及表层浮游生物、深层海底沉积物、鲸尸及土壤相关研究的数据进行比较宏基因组学分析后，发现了若干值得关注的趋势。本研究关联的核糖体RNA序列编号为HM103388-HM103902。