Data for: Top-down control and species composition non-linearly influence the short-term response of experimental food webs to a nutrient pulse perturbation

Extreme weather events intensify with global change and frequently disturb aquatic ecosystems via e.g., nutrient pulses through surface runoff. While there is accumulating evidence that the associated short-term responses of plankton communities depend on their top-down control (TC), the actual shape of TC-community response relationships remains unclear. We therefore conducted 24 flow-through (chemostat) experiments to investigate algal and rotifer biomass responses to a nutrient pulse along a gradient of TC values. To generate this gradient, we used a trait-based method instead of varying the inoculation species densities. We complemented our analysis by estimating non-measured nutrient dynamics and feeding interactions via Bayesian modelling. We found support for hump-shaped relationships. At low TC, algal species were strongly nutrient-depleted, limiting their immediate growth potential after the pulse, and thereby one of the rotifer species. At high TC, algae were not nutrient-limi..., The aim of this study is to investigate the response in biovolume of phytoplankton (algae) and zooplankton (rotifers) after a nutrient pulse perturbation. We notably test two types of relationships:

the hump-shaped relationship between biovolume responses of algae and the top-down control at the pulse perturbation
the hump-shaped relationship between biovolume responses of rotifers and the top-down control at the pulse perturbation

In the following, you can find a summary of the methods. Please see the published study for more details.
We cultivated the four algal species Chlamydomonas reinhardtii (strain No. SAG 11-32b), Monoraphidium minutum (SAG 243-1), Chlorella vulgaris (SAG 211-11b), and Cryptomonas sp. (SAG 26-80), and the three herbivorous rotifer species Brachionus calyciflorus sensu strictu (isolated from the Milwaukee Harbor, Wisconsin, USA), Cephalodella sp. (obtained from Florian Altermatt, Campus pond), and Lecane sp. (isolated by Christina Schirmer, Löschteich Golm, Pot..., , # Data for: Top-down control and species composition non-linearly influence the short-term response of experimental food webs to a nutrient pulse perturbation

[https://doi.org/10.5061/dryad.51c59zwj5](https://doi.org/10.5061/dryad.51c59zwj5)

## Description of the data and file structure

# Information about the datasets and the code

The aim of this study is to investigate the response in biovolume of phytoplankton (algae) and zooplankton (rotifers) after a nutrient pulse perturbation. We notably test two types of relationships:

* the hump-shaped relationship between biovolume responses of algae and the top-down control at the pulse perturbation
* the hump-shaped relationship between biovolume responses of rotifers and the top-down control at the pulse perturbation

First, we conducted experiments and then inferred the experimental data via Bayesian inference. Data analysis was performed in R 4.5.0 and RStudio environment using notably the libraries tidyverse, PKNCA, cmdstanr, brms, ...,

极端天气事件随全球变化加剧，常通过地表径流引发的养分脉冲等方式干扰水生生态系统。尽管已有越来越多证据表明，浮游生物群落的相关短期响应依赖于其下行控制（top-down control, TC），但TC与群落响应之间关系的具体形态仍不明确。为此，我们开展了24组流水式（恒化器，chemostat）实验，探究在下行控制梯度下，藻类与轮虫生物量对养分脉冲的响应。为构建该下行控制梯度，我们采用基于功能性状的方法，而非通过调整接种物种密度实现。我们通过贝叶斯建模（Bayesian modelling）估算未直接测量的养分动态与捕食互作，以此补充分析内容。我们的结果支持驼峰型关系的存在。在低下行控制水平下，藻类物种受到强烈的养分限制，削弱了其在养分脉冲后的即时生长潜力，进而影响其中一种轮虫物种；在高下行控制水平下，藻类并未受到养分限...
本研究旨在探究浮游植物（藻类）与浮游动物（轮虫）的生物体积在养分脉冲扰动后的响应特征，重点检验两类关系：
1. 藻类生物体积响应与养分脉冲扰动下的下行控制之间的驼峰型关系
2. 轮虫生物体积响应与养分脉冲扰动下的下行控制之间的驼峰型关系
下文为实验方法概述，详细内容请参见已发表的研究论文。
我们共培养了4种藻类：莱茵衣藻（*Chlamydomonas reinhardtii*，菌株号SAG 11-32b）、纤细单歧藻（*Monoraphidium minutum*，SAG 243-1）、普通小球藻（*Chlorella vulgaris*，SAG 211-11b）以及隐藻属物种（*Cryptomonas* sp.，SAG 26-80）；同时培养了3种草食性轮虫物种：严格型臂尾轮虫（*Brachionus calyciflorus* sensu strictu，分离自美国威斯康星州密尔沃基港）、头叶轮虫属物种（*Cephalodella* sp.，由Florian Altermatt从校园池塘提供）以及肢轮虫属物种（*Lecane* sp.，由Christina Schirmer从德国戈尔姆的勒施泰希池塘（Löschteich Golm）分离...

# 数据集：下行控制与物种组成非线性调控实验食物网对养分脉冲扰动的短期响应
[https://doi.org/10.5061/dryad.51c59zwj5](https://doi.org/10.5061/dryad.51c59zwj5)

## 数据与文件结构说明

## 数据集与代码说明
本研究旨在探究浮游植物（藻类）与浮游动物（轮虫）的生物体积在养分脉冲扰动后的响应特征，重点检验两类关系：
* 藻类生物体积响应与养分脉冲扰动下的下行控制之间的驼峰型关系
* 轮虫生物体积响应与养分脉冲扰动下的下行控制之间的驼峰型关系
首先我们开展实验，并通过贝叶斯推断对实验数据进行反演。数据分析在R 4.5.0与RStudio环境中完成，主要使用了tidyverse、PKNCA、cmdstanr、brms等R包...