Methylation levels in H3.3 mutant hematopoietic stem cells.

Reduced Representation Bisulfite Sequencing for mouse in vivo hematopoietic stem cells, 3 conditions: wildtype, expressing dominant negative mutant H3.3 at lysine 9 (K9M) or dominant negative H3.3 at lysine 36 (K36M). DNA was extracted, digested with Msp1, bisulfite-converted and sequenced to assess DNA methylation levels.

本数据集针对小鼠体内造血干细胞开展简化代表性亚硫酸氢盐测序（Reduced Representation Bisulfite Sequencing），共设置3组实验条件：野生型样本，以及分别表达赖氨酸9位（K9）发生K9M突变的显性负突变型组蛋白H3.3、赖氨酸36位（K36）发生K36M突变的显性负突变型组蛋白H3.3的样本。实验流程包括提取基因组DNA，经Msp1限制性内切酶酶切、亚硫酸氢盐转化处理后进行测序，以此检测样本的DNA甲基化水平。