Gene expression profile of OSSL_325096 treated myeloma cells. Gene expression profile of OSSL_325096 treated myeloma cells

OSSL_325096 is a compound that acts as p97/VCP inhibitor and induces apoptosis in myeloma cells. Therefore, we treated KMS12PE and KMS11 myeloma cell lines with OSSL_325096 or DMSO and compare gene expression levels to elucidate the mechanisms of apoptosis. Overall design: We treated KMS12PE and KMS11 myeloma cell lines with 1 μM OSSL_325096 or DMSO. RNA was extracted and purified using Trizol reagent (Thermo Fisher Scientific) and an RNeasy Mini Kit (Qiagen). Primary RNA-sequencing was performed by Novogene (Beijing, China). Raw sequence data was mapped to hg19 and expression values were determined as RPKM using Biomedical Genomics Workbench 5 software (QIAGEN Bioinformatics, Venlo, Netherlands). Then gene expression levels in two myeloma cell lines were compared using Biomedical Genomics Workbench 5 software.

OSSL_325096是一种p97/VCP抑制剂类化合物，可诱导骨髓瘤细胞凋亡。为阐明其介导细胞凋亡的分子机制，我们分别以OSSL_325096与二甲基亚砜（DMSO）处理KMS12PE和KMS11骨髓瘤细胞系，通过比较二者的基因表达水平开展相关研究。实验整体设计：我们采用1 μM浓度的OSSL_325096或DMSO处理KMS12PE与KMS11骨髓瘤细胞系。使用Trizol试剂（赛默飞世尔科技，Thermo Fisher Scientific）与RNeasy Mini Kit（凯杰，Qiagen）提取并纯化总RNA。原始RNA测序由北京诺禾致源（Novogene，中国北京）完成。将原始测序数据比对至人类参考基因组hg19，并采用Biomedical Genomics Workbench 5软件（QIAGEN Bioinformatics，荷兰文洛）以每百万比对读段每千碱基片段数（Reads Per Kilobase per Million mapped reads，RPKM）计算基因表达量。随后，借助Biomedical Genomics Workbench 5软件比较两种骨髓瘤细胞系的基因表达水平。