通过单细胞测序揭示华坦解玉颗粒治疗帕金森病的治疗靶点、机制和异质性

在本研究中，对对照组、PD 模型和治疗组的小鼠进行单细胞测序。在对单细胞数据进行质量控制和缩小后，将它们分为不同的细胞簇。之后，从中鉴定出不同的细胞类型。此外，采用对照组和模型组中 DEGs1 与模型组和处理组中 DEGs2 的交集得到相交的 DEGs。此后，通过与中药活性成分的药物靶点交叉，筛选出关键药物靶点。基于 PPI 网络中的拓扑结构分析，确定了关键基因。将关键基因高表达的细胞类型作为关键细胞。此外，关键细胞受到细胞通讯和提出的时间序列的影响。最后，关键细胞也被分为不同的亚型。

In this study, single-cell RNA sequencing was conducted on mice from the control group, PD model group, and treatment group. Following quality control and dimensionality reduction of the single-cell data, the cells were partitioned into distinct cell clusters, based on which distinct cell types were subsequently identified. Additionally, overlapping differentially expressed genes (DEGs) were obtained by intersecting DEGs1 (from the control vs. model groups) and DEGs2 (from the model vs. treatment groups). Thereafter, key drug targets were screened by cross-referencing with the drug targets of active ingredients from traditional Chinese medicine (TCM). Key genes were then identified through topological structure analysis of the protein-protein interaction (PPI) network. Cell types exhibiting high expression of these key genes were designated as key cells. Moreover, key cells were analyzed in association with cell communication and the proposed time-series datasets. Finally, key cells were further subdivided into distinct subtypes.