Comparison of gene expression in AhR WT (+/+) and AhR KO (-/-) primary mouse keratinocytes

The AhR is a ligand activated transcription factor that may be important in normal skin physiology. We compared gene expression profiles between AhR Wt and AhR KO primary mouse keratinocyte cultures. We identified 391 genes that were differentially expressed with a 1.5 fold cutoff and p<.05, and identified the AhR as an important regulator of genes involved in normal epidermal differentiation. AhR Wt primary keratinocyte cultures (n=4) were compared with AhR KO primary keratinocyte cultures (n=3)

芳香烃受体（Aryl Hydrocarbon Receptor，AhR）是一种配体激活的转录因子，其在皮肤正常生理过程中可能发挥重要调控作用。本研究对比了AhR野生型（AhR Wt）与AhR敲除型（AhR KO）原代小鼠角质形成细胞培养物的基因表达谱。通过1.5倍表达差异阈值与p<0.05的显著性筛选标准，本研究共鉴定出391个差异表达基因，并证实AhR是参与表皮正常分化进程的关键调控因子。本研究纳入的AhR野生型原代小鼠角质形成细胞培养样本共4份（n=4），敲除型样本共3份（n=3）。