Stradard protein and yeast top-down data

We developed a novel dissociation strategy in the data acquisition process, the Mesh strategy to dissociate multiple charge states of one proteoform with stepped HCD (high collision energy dissociation). We proved that the ‘Mesh’ strategy could generate fragments with improved sequence coverage and it could also improve identification rate in top-down proteomics analysis for complicated samples. The strategy is implemented within an an open source real-time instrument control software program called MetaDrive to perform real time deconvolution and precursor selection.

我们在数据采集流程中开发了一种全新的解离策略——"Mesh策略"，该策略采用阶梯式高能碰撞解离（high collision energy dissociation，HCD）技术，对单个蛋白质变体（proteoform）的多种电荷态进行解离。实验证实，"Mesh策略"不仅可生成序列覆盖度更优的质谱片段，还能提升复杂样品自上而下蛋白质组学（top-down proteomics）分析中的蛋白质鉴定率。本策略已集成至一款名为MetaDrive的开源实时仪器控制软件中，用于实现实时解卷积与前体选择。