The DNA methylation landscape of human early embryos

Our data provided a genome-wide DNA methylation landscape of human early development embryos, including human MII oocytes, sperm, zygotes, 2-cell to 8-cell embryos, morula, blastocyst and postimplantation embryos at single base resolution. Overall design: In total, 44 samples including biological and technical replicates, from 12 different human embryo development stages were analyzed, including two metaphase II oocytes, two zygotes, three first polar bodies, two second polar bodies, four sperm samples, two 2-cell-stage embryos, two 4-cell-stage embryos, three 8-cell-stage embryos, three morulae, three inner cell masses (ICMs) and three trophectoderms (TEs) seperated from late blastocysts, and three post-implantation embryos. In addition, 12 different human embryo development stages were analyzed, including metaphase II oocytes, zygotes, first polar bodies, second polar bodies, sperm samples, 2-cell-stage embryos, 4-cell-stage embryos, 8-cell-stage embryos, morulae, inner cell masses (ICMs) and trophectoderms (TEs) seperated from late blastocysts, and post-implantation embryos.

我们的数据集提供了人类早期发育胚胎的全基因组DNA甲基化图谱，涵盖MII期卵母细胞、精子、受精卵、2细胞至8细胞胚胎、桑葚胚、囊胚及着床后胚胎，且分辨率达到单碱基水平。
整体设计：共分析了来自12个不同人类胚胎发育阶段的44个样本（含生物学重复与技术重复），包括2个MII期卵母细胞、2个受精卵、3个第一极体、2个第二极体、4个精子样本、2个2细胞期胚胎、2个4细胞期胚胎、3个8细胞期胚胎、3个桑葚胚、3个分离自晚期囊胚的内细胞团（ICM）、3个分离自晚期囊胚的滋养外胚层（TE），以及3个着床后胚胎。