Table_1_Regulation of soybean drought response by mepiquat chloride pretreatment.xlsx

IntroductionSoybean is the world’s most important cultivated crop, and drought can affect their growth and, eventually, yields. Foliar application of mepiquat chloride (MC) can potentially alleviate the damage caused by drought stress in plants; however, the mechanism of MC regulation of soybean drought response has not been studied.
MethodsThis study investigated the mechanism of soybean drought response regulation by mepiquat chloride in two varieties of soybean, sensitive Heinong 65 (HN65) and drought-tolerant Heinong44 (HN44), under three treatment scenarios, normal, drought stress, and drought stress + MC conditions.
Results and discussionMC promoted dry matter accumulation under drought stress, reduced plant height, decreased antioxidant enzyme activity, and significantly decreased malondialdehyde content. The light capture processes, photosystems I and II, were inhibited; however, accumulation and upregulation of several amino acids and flavonoids by MC was observed. Multi-omics joint analysis indicated 2-oxocarboxylic acid metabolism and isoflavone biosynthetic pathways to be the core pathways by which MC regulated soybean drought response. Candidate genes such as LOC100816177, SOMT-2, LOC100784120, LOC100797504, LOC100794610, and LOC100819853 were identified to be crucial for the drought resistance of soybeans. Finally, a model was constructed to systematically describe the regulatory mechanism of MC application in soybean under drought stress. This study fills the research gap of MC in the field of soybean resistance.
ConclusionNETs can promote gastric cancer metastasis by initiating COX-2 through TLR2, and COX-2 may become a target for gastric cancer immunotherapy.

引言 大豆是全球最重要的栽培作物，干旱胁迫会影响其生长进程，并最终降低产量。叶面喷施甲哌鎓（mepiquat chloride, MC）可缓解植物遭受干旱胁迫时受到的损伤，但目前关于MC调控大豆干旱响应的分子机制尚未得到研究。
方法 本研究以两个大豆品种——干旱敏感型品种黑农65（Heinong 65, HN65）和耐旱型品种黑农44（Heinong 44, HN44）为实验材料，设置正常栽培、干旱胁迫、干旱胁迫+MC处理三种实验组别，探究甲哌鎓调控大豆干旱响应的具体机制。
结果与讨论 干旱胁迫条件下，甲哌鎓可促进大豆干物质积累、降低株高、下调抗氧化酶活性，并显著降低丙二醛含量。光捕获过程以及光系统I、II的活性受到抑制，但甲哌鎓处理可促进多种氨基酸与黄酮类物质的积累并上调其表达水平。多组学联合分析显示，2-氧羧酸代谢途径与异黄酮生物合成途径是甲哌鎓调控大豆干旱响应的核心通路。本研究鉴定出LOC100816177、SOMT-2、LOC100784120、LOC100797504、LOC100794610及LOC100819853等与大豆抗旱性密切相关的候选基因。最后，本研究构建了调控模型以系统阐述干旱胁迫下叶面喷施甲哌鎓对大豆的调控机制，填补了甲哌鎓在大豆抗逆领域的研究空白。
结论 中性粒细胞胞外陷阱（NETs）可通过Toll样受体2（TLR2）激活COX-2，进而促进胃癌转移，COX-2或可成为胃癌免疫治疗的潜在靶点。