Mapping of a resistance gene for Albugo laibachii from the Arabidopsis accession Sf-2

In a cross of Sf-2xMAGIC329, resistance segregated 3:1 (R:S) in the F2 generation, suggesting a single gene/locus confers resistance. To map the gene responsible, 200 susceptible individuals from the F2 were collected and DNA extracted. This pooled "bulk susceptible" DNA was captured using the BrRenSeq v2 bait library to select for NLR encoding loci and sequenced on the MiSeq platform as one of a set of pooled barcoded samples (TGAC-ENQ-1124/PRO1067).

在Sf-2xMAGIC329的杂交组合中，F2代的抗病性以3:1（抗感，R:S）的比例分离，提示该抗病性由单基因/单位点赋予。为定位该抗病相关基因，研究人员收集了200株F2代感病个体并提取其基因组DNA。将该感病混合池DNA使用BrRenSeq v2诱饵文库进行靶向捕获，以富集编码核苷酸结合富亮氨酸重复序列（NLR）的基因位点，并作为一组带条形码混合样本（TGAC-ENQ-1124/PRO1067）中的一个，在MiSeq测序平台上完成测序。