Gene expression microarray analysis of early oxygen-induced retinopathy (OIR). Rattus norvegicus

Different inbred strains of rats differ in their susceptibility to OIR, an animal model of human retinopathy of prematurity. We examined gene expression profiles in Fischer 344 (F344, resistant to OIR) and Sprague Dawley (SD, susceptible to OIR) rats at the early time point of day 3 to identifying gene pathways related to the underlying genetic cause of phenotypic differences between strains. Overall design: To examine gene expression changes in rats strains which are resistant and susceptible to OIR, four different experimental conditions were analysed: F344 cyclic hyperoxia (O2) exposed, F344 room air (RA) exposed, SD O2 exposed and SD RA exposed. A minimum of two samples of pooled RNA, comprising of 3 individual rats from 2 separate litters, was used for each experimental condition. Pooled RNA from different rats were used as biological replicates. An additional sample for F344 O2 and SD RA rats was included on the basis of the principal components analysis from the initial 8 samples. Pooled RNA from age-matched room air-exposed rats were used as controls.

不同近交系大鼠对氧诱导视网膜病变（Oxygen-Induced Retinopathy, OIR）的易感性存在差异，而OIR是人类早产儿视网膜病变的动物模型。本研究于第3天这一早时间点，对OIR抗性品系Fischer 344（F344）与易感品系Sprague Dawley（SD）大鼠的基因表达谱进行检测，旨在鉴定与不同品系间表型差异的潜在遗传病因相关的基因通路。总体实验设计：为探究抗OIR与易感OIR大鼠品系的基因表达变化，本研究共分析4组实验条件：F344品系周期性高氧（O₂）暴露组、F344品系常氧（Room Air, RA）暴露组、SD品系O₂暴露组及SD品系RA暴露组。每组实验条件至少采用2份混合RNA样本，每份样本由2个独立窝的3只个体大鼠的RNA混合制备。以不同大鼠来源的混合RNA作为生物学重复。基于初始8份样本的主成分分析结果，本研究额外补充了F344 O₂暴露组与SD RA暴露组的各1份样本。以年龄匹配的常氧暴露大鼠的混合RNA作为对照。