An interpretation of the genetic polymorphism and population genetic background of Ankang Han population via a novel InDel panel

In this research, genotyping data of 43 InDel loci in 311 Han individuals in Ankang city, Shaanxi province, China were detected using a self-developed five-dye multiplex amplification panel. The allelic frequencies and forensic parameters of all InDel loci were calculated. The combined power of discrimination and probability of exclusion values were 0.999 999 999 999 999 998 827 39 and 0.999 887 424, respectively, which demonstrated that this 43-InDel panel was powerful for individual identifications in Ankang Han population. Moreover, genetic distances, pairwise <i>F_ST</i> values, principal component analyses, phylogenetic trees and STRUCTURE analyses were performed to investigate the genetic affinities between Ankang Han and reference groups. Population genetic investigations indicated that Ankang Han population had a close genetic relationship with Southern Han population compared with other reference groups.

本研究采用自主研发的五染料多重扩增分型检测体系，对中国陕西省安康市311名汉族个体的43个插入缺失（InDel）位点进行了基因分型检测。本研究计算了所有InDel位点的等位基因频率及法医学参数，其累积个体识别率与累积非父排除概率分别为0.99999999999999999882739与0.999887424，结果表明该43-InDel分型体系可有效应用于安康汉族人群的个体识别。此外，本研究通过计算遗传距离、成对遗传分化系数（F_ST）值，开展主成分分析、系统发育树构建及群体结构（STRUCTURE）分析，以探究安康汉族与参考人群组间的遗传亲缘关系。群体遗传学分析结果显示，相较于其他参考人群，安康汉族人群与南方汉族人群的遗传亲缘关系更为紧密。