Human aryl hydrocarbon receptor (AhR) signaling responses to MC252 oil fractions in YCM3 yeast

These are a series of comparative responses of human aryl hydrocarbon receptor (AhR) signaling to MC252 oil fractions. The material was fractionated oil by D. Pangeni and E. Overton, collaborators at LSU using a silica gel chromatography method with hexane as the starting solvent and mixing with dichlormethane up to 50% final volume to resolve the aromatic hydrocarbons. 50 ul aliquots were dried and sent to the Miller lab for human AhR signaling and other toxicity testing. YCM3 yeast was exposed to varying dilutions of the 27 oil fractions and assessed for AhR signaling with a lacZ reporter assay. The greater the dilution that retains signing ability, the more potent and/or abundant the PAH ligand(s) are in the sample. Two strong peaks of activity are seen for fractions 17-18 and 20-24. These fractions contain different PAHs indicated in the chart at the end of the data. Compounds of interest in the fractions include methyl-substituted chrysenes.

这是一系列人类芳香烃受体（aryl hydrocarbon receptor, AhR）信号通路对MC252石油馏分响应的比较数据。该材料为MC252石油馏分，由路易斯安那州立大学（LSU）合作者D. Pangeni与E. Overton采用硅胶色谱法制备：以己烷为初始溶剂，逐步加入二氯甲烷（dichlormethane）至最终体积占比50%，以分离芳香烃。取50微升（μl）等分试样干燥后，送往Miller实验室进行人类AhR信号通路及其他毒性测试。将YCM3酵母暴露于27种石油馏分的不同稀释液中，通过lacZ报告基因测定法（lacZ reporter assay）评估AhR信号通路活性。能维持信号活性的稀释度越高，样本中多环芳烃（PAH）配体的效力越强和/或含量越丰富。17-18号及20-24号馏分呈现出两个显著的活性峰值。这些馏分所含的不同PAH详见数据末尾的图表。馏分中值得关注的化合物包括甲基取代䓛类。