RNA sequencing of mouse placenta in control and protein restricted pregnancies

We used RNA sequencing to identify differentially expressed genes in whole mouse placentas. Pregnant mice were fed either a 20% or 6% protein (isocaloric) two-weeks before mating and throughout pregnancy. Placental lysates are derived from C57BL/6 conceptuses at E16.5 of gestation. Examination of RNA expression in mouse placenta at E16.5. Samples were same-sex litter mates from n=5 pregnancies.

本研究采用RNA测序（RNA sequencing）技术鉴定完整小鼠胎盘中的差异表达基因。在交配前两周及整个妊娠阶段，受试孕鼠被分别饲喂等热量的20%蛋白质饲料与6%蛋白质饲料，其胎盘裂解物取自妊娠第16.5天（E16.5）的C57BL/6品系孕体。本研究针对妊娠第16.5天的小鼠胎盘RNA表达水平开展检测，所有样本均来自5次妊娠（n=5）的同性同窝幼崽。