Identifying ASCL1 target genes in primary GBM stem cell cultures [RNA-seq]. Homo sapiens

ASCL1 mediates neuronal differentiation of GBM stem cell (GSC) cultures. We sought to identify targets of ASCL1 in primary human GSC cultures. In this dataset, we include RNA-seq data obtained from GSC cultures harbouring a CRISPR-deletion of ASCL1. We assessed differential gene expression between control and GSC cultures induced to overexpress ASCL1 after 7 days of doxycycline treatment. Overall design: Glioblastoma cells with a genetic knockout of ASCL1 is compared to genetic knockout of ASCL1 overexpressing ASCL1 (n = 3 biological replicates; total = 6 samples)

ASCL1介导胶质母细胞瘤干细胞（GBM stem cell, GSC）培养物的神经元分化。本研究旨在鉴定原代人源GSC培养物中ASCL1的靶基因。本数据集包含携带ASCL1 CRISPR敲除的GSC培养物的RNA测序（RNA-seq）数据。我们对对照组与经多西环素（doxycycline）处理7天后被诱导过表达ASCL1的GSC培养物开展了差异基因表达分析。总体实验设计：将携带ASCL1基因敲除的胶质母细胞瘤细胞，与携带ASCL1基因敲除且过表达ASCL1的胶质母细胞瘤细胞进行对比（每组3次生物学重复，总计6个样本）。