Role of ST2 expession in regulatory Tcells on muscle regneration. Mus musculus

We describe the effects of Treg ST2 expression on muscle regeneration. We compare regneration from cryo-injured tibialis anterior muscle from Foxp3-Cre/ST2-flox vs Foxp3-Cre mice 8 days post-injury. These analyses from whole muscle provide insight into the role of muscle Tregs in the context of regeneration. Overall design: The tibialis anterior muscle from male Foxp3-Cre/ST2 flox or Foxp3-Cre/ST2 WT C57BL/6 mice (2 months old) were cryo-injured using a liquid nitrogen chilled metal probe for 8 seconds. 8 days post-injury whole muscle was harvested and snap frozen in liquid nitrogen. Whole muscle was then homogenized directly in TriZol and gene expression profiling was performed on Affymetrix Mouse Gene 1.0 ST Arrays.

本研究阐明了调节性T细胞（Treg）的ST2表达对肌肉再生的影响。我们对比了损伤后8天时，Foxp3-Cre/ST2条件性敲除（flox）与Foxp3-Cre野生型（WT）小鼠的冷冻损伤胫骨前肌的再生水平。本次基于整块肌肉的分析，可为解析肌肉驻留Treg在肌肉再生过程中的功能提供参考。
实验设计：选取2月龄雄性C57BL/6背景的Foxp3-Cre/ST2 flox或Foxp3-Cre/ST2 WT小鼠，使用液氮预冷的金属探针对其胫骨前肌实施8秒的冷冻损伤。损伤后8天，收取整块肌肉并于液氮中快速冷冻。随后将整块肌肉直接在Trizol试剂中匀浆，并采用Affymetrix小鼠基因1.0 ST芯片阵列开展基因表达谱分析。