Time course of MEK inhibition. Homo sapiens

This study used microarray expression analysis to identify global changes in transcript alteration in response to MEK inhibition. Genes under ERK control were identified in a representative V600E BRAF cell line as a function of time following exposure to a small molecule inhibitor of MEK. Keywords: Time course Overall design: SkMel-28 cells growing in culture were treated with the MEK inhibitor PD0325901 for 2, 8, or 24 hours. Changes in RNA compared to reference (time =0) were measured using microarray analysis.

本研究采用微阵列（microarray）表达谱分析，鉴定响应丝裂原活化蛋白激酶激酶（Mitogen-activated protein kinase kinase, MEK）抑制时的转录本变化整体特征。本研究以具有代表性的V600E BRAF突变型细胞系为模型，以暴露于MEK小分子抑制剂后的时间为观测变量，鉴定得到受细胞外调节蛋白激酶（Extracellular signal-regulated kinase, ERK）调控的基因。
关键词：时间进程
实验整体设计：将体外培养的SkMel-28细胞用MEK抑制剂PD0325901分别处理2、8或24小时，以处理0小时的样本作为参照，通过微阵列分析检测各组与参照相比的RNA表达变化。