Comparison of plasma cytokine or chemokine levels found in the two groups of patients.

The cytokine and chemokine concentrations in the serum of 13 Group 1 and 54 Group 2 patients were determined by flow cytometry (using CBA flex set®). All the available plasma samples were tested at the same time and the differences between concentrations were determined by non parametric 1 way median Tests between Group 1 or Group 2 patients. 10 control individuals (healthy malaria naïve individuals) were also tested and, as expected, cytokine or chemokine concentrations differed from those found in patients, Significance levels are indicated by star symbols as follows: * p<0.05, ** p<0.01 and *** p<0.001.

本研究采用流式细胞术（flow cytometry）结合CBA flex set®试剂盒，对13名第1组患者与54名第2组患者的血清细胞因子及趋化因子浓度进行检测。所有可用的血浆样本均于同一批次完成检测，第1组与第2组患者的浓度差异采用非参数单因素中位数检验进行分析。另纳入10名健康无疟史个体（healthy malaria naïve individuals）作为对照，其细胞因子与趋化因子浓度与患者组存在显著差异，该结果符合预期。显著性水平通过星号标注，具体规则如下：* 代表p<0.05，** 代表p<0.01，*** 代表p<0.001。