CIL:38604

This total internal reflection (TIRF) image of unconverted tdEos-paxillin (green) and PsCFP20-zyxin (red) demonstrates that these two proteins are almost completely colocalized when observed with diffraction limited optics. The true localization pattern is shown in the corresponding photoactivation localization microscopy (PALM) image, CIL 38602. Note the differential interference contrast (DIC) image corresponding to the same image field is also available as CIL 38603. Bar is 2 microns. Image made available by Catherine and James Galbraith and corresponds to Figure 4 in PNAS U S A. 2007 Dec 18;104(51):20308-13.

本幅未转换的tdEos标记桩蛋白（tdEos-paxillin，绿色）与PsCFP20标记斑联蛋白（PsCFP20-zyxin，红色）的全内反射（total internal reflection, TIRF）图像显示，在衍射极限光学系统（diffraction limited optics）下观测时，这两种蛋白几乎完全共定位。对应的光激活定位显微术（photoactivation localization microscopy, PALM）图像（CIL 38602）展示了其真实的定位模式。需注意，同一场景的差分干涉衬度（differential interference contrast, DIC）图像也可通过CIL 38603获取。图像比例尺为2微米。本图像由Catherine与James Galbraith提供，对应《美国国家科学院院刊》（PNAS USA）2007年12月18日，第104卷第51期，第20308-20313页的图4。