Physiological responses of seeds from full-sib guava families to different substrate temperatures

Abstract Guava is an important fruit in Brazil and worldwide. However, research has yet to advance on its breeding and seed technology, an essential factor for the development of productive genotypes with germination potential which would support the continuity of breeding programs. In search of a methodology for routine analysis testing of guava seeds under genetic control, this study examined the effect of temperature and substrate on the germination of guava seeds from full-sib families. The experiment was laid out in a completely randomized design with a 5 × 4 factorial arrangement consisting of the combination of five temperatures (25, 30, 35, 20-30 and 25-35 °C) and four substrates (paper roll, on-paper, in-sand and on-sand), with eight replicates of 50 seeds. Germination, germination speed index and live ungerminated seeds were evaluated. The alternating temperatures of 20-30 and 25-35 °C and the paper roll, on-paper and in-sand substrates are adequate conditions for germination tests with guava seeds.

摘要 番石榴（Guava）是巴西乃至全球范围内的重要果品。然而，当前其育种与种子技术的研究仍有待深化——该技术是培育具备发芽潜力的高产基因型、支撑育种项目可持续开展的核心要素。为探寻番石榴种子遗传背景下常规发芽检测的标准化方法，本研究以全同胞家系的番石榴种子为材料，探究了温度与基质对其发芽的影响。本实验采用5×4因子组合的完全随机设计，设置5个温度梯度（25、30、35、20-30及25-35 ℃）与4种发芽基质（卷纸法、纸上法、沙中法及沙面法），每个处理设置8次重复，每次重复使用50粒种子。本次试验测定的指标包括发芽率、发芽速度指数与存活未发芽种子数。结果表明，20-30 ℃与25-35 ℃的变温条件，以及卷纸法、纸上法与沙中法三种基质，均适用于番石榴种子的发芽检测试验。