Glucose Glycerol fed-batch fermentation under elevated pCO2

In this work we studied the effect of elevated pCO2 and high substrate concentration on the product spectrum of mixed culture anaerobic degradation of a mixed substrate composed of glycerol and glucose. In parallel, we investigated the independent conversion of the employed substrates at elevated pCO2 and the mixture with a non-reactive headspace (nitrogen). We employed fed-batch operation regime in order to evaluate the effects of the biomass adaptation to the pressurized incubation in product formation and substrate consumption.

本研究探究了升高的二氧化碳分压（pCO2）与高底物浓度对混合菌群厌氧降解甘油和葡萄糖混合底物的产物谱的影响。与此同时，本研究还考察了所用底物在升高的pCO2条件下的单独转化过程，以及顶空填充惰性气体（氮气，nitrogen）的混合底物转化过程。本研究采用补料分批操作模式，旨在评估微生物群落适配加压培养对产物生成与底物消耗的影响。