Flow Magnetic Tweezers: Gyrase dynamics in presence of 20 uM drug Ciprofloxacin

The video contains a whole field from a force spectroscopy experiment called Flow Magnetic Tweezers (FMT). It shows E. coli DNA Gyrase manipulating DNA topology by relaxing positive and introducing negative coils in presence of 20 uM of Ciprofloxacin, reaction of Gyrase to external torque and eventual wash with SDS.

本视频展示了一项名为流式磁镊（Flow Magnetic Tweezers, FMT）的力谱学实验的完整实验过程。实验内容为：大肠杆菌DNA旋转酶（E. coli DNA Gyrase）在20微摩尔环丙沙星（Ciprofloxacin）存在的条件下，通过松弛正超螺旋并引入负超螺旋来调控DNA拓扑结构；同时观测该旋转酶对外部扭矩的响应，以及最终用十二烷基硫酸钠（SDS）完成冲洗的全过程。