Bordetella bronchiseptica strain:GD14469_1 Genome sequencing

This genome represents a Bordetella bronchiseptica isolate, which was initially misidentified as Bordetella pertussis through molecular diagnostic methods. Subsequent culture, MALDI-TOF mass spectrometry and genomic sequencing of the isolate confirmed the correct identification as B. bronchiseptica. This case underscores the importance of considering potential caveats when using molecular diagnostic assays for detecting Bordetella pertussis, as misidentifications can occur due to similarities between species. It highlights the necessity of employing complementary diagnostic techniques, to ensure accurate pathogen identification, especially in clinical settings where accurate diagnosis is critical for appropriate treatment and epidemiological surveillance.

本基因组对应一株支气管败血波氏杆菌（Bordetella bronchiseptica）分离株，该菌株最初通过分子诊断方法被误鉴定为百日咳博德特氏菌（Bordetella pertussis）。后续对该分离株开展的培养、基质辅助激光解吸电离飞行时间质谱（MALDI-TOF mass spectrometry）检测及基因组测序，确认其正确鉴定结果为支气管败血波氏杆菌。本案例凸显了在利用分子诊断检测手段开展百日咳博德特氏菌检测时，需充分考量潜在局限性的重要性——由于物种间的遗传相似性，可能出现鉴定错误。该案例同时强调，需采用互补性诊断技术以确保病原体的精准鉴定，这在临床场景中尤为关键：准确诊断对于制定合理治疗方案及开展流行病学监测均具有核心意义。