Enigma (CG9006) RNAi vs control RNAi in Drosophila Kc-167 cells.

5 day RNAi treatment to knockdown Enigma, CG9006, a Drosophila mitochondrial protein with homology to acyl-CoA dehydrogenases. Keywords: Genetic modification Kc-167 cells, 5 day treatment with Enigma-specific RNAi or beta-lactamase control RNAi. Specifics:1.6X10^6 cells in 0.5mL of serum-free Sang's M3 media incubated for 1 hour with 20ug of dsRNA, subsequently equal volume of M3 media containing 10% serum was added. Cells harvested after 5 days. RNAi efficiency measured by immunoblot for Enigma.

采用为期5天的RNA干扰（RNAi）处理，对与酰基辅酶A脱氢酶（acyl-CoA dehydrogenases）同源的果蝇（Drosophila）线粒体蛋白Enigma、CG9006进行敲低。
关键词：遗传修饰Kc-167细胞、采用Enigma特异性RNA干扰或β-内酰胺酶（beta-lactamase）对照RNA干扰进行为期5天的处理。
具体实验步骤：将1.6×10^6个细胞置于0.5mL无血清Sang's M3培养基中，与20μg双链RNA（dsRNA）孵育1小时，随后加入等体积含10%血清的M3培养基。于5天后收集细胞；通过免疫印迹（immunoblot）实验检测Enigma以评估RNA干扰效率。