Single-nucleotide resolution profiling of N6-methyladenosine in SETD2 knockdown HepG2 cells [m6A miCLIP-seq]. Single-nucleotide resolution profiling of N6-methyladenosine in SETD2 knockdown HepG2 cells [m6A miCLIP-seq]

To understand the global effect of H3K36me3 on m6A modification, we compared the m6A profiling in SETD2 knockdown and control HepG2 cells by miCLIP-seq, and found the depletion of H3K36me3 by SETD2 silencing globally reduced m6A in the human transcriptome. Overall design: miCLIP-seq in HepG2 cells with stable expressed SETD2 or non-specific control shRNAs.

为阐明H3K36me3（组蛋白H3第36位赖氨酸三甲基化）对m6A（N6-甲基腺嘌呤）修饰的全局调控效应，我们采用miCLIP-seq技术对比了SETD2敲低组与对照组HepG2细胞的m6A图谱，发现通过SETD2沉默实现的H3K36me3耗竭可在全局水平上降低人类转录组中的m6A修饰水平。总体实验设计：在稳定表达SETD2短发夹RNA（shRNA）或非特异性对照shRNA的HepG2细胞中开展miCLIP-seq实验。