The activity of compounds, including two clinically used drugs, against T. cruzi infection and replication.

1) Infection of 3T3 fibroblasts in vitro was determined with a well-established intracellular infection assay [7]. These results were compared to the replication of T. cruzi measured in 3T3 cells utilising a combination of Hoechst and EdU. For (2), the maximum activity demonstrated with POSA (Emax) against T. cruzi was 75%, therefore the IC50 value was based on a sub-optimal effect. 3) Significance pmax values. NR = not replicating. Cmpd = compound. CF = clemastine fumarate, POSA = posaconazole, NFX = nifurtimox, BZ = benznidazole.

1) 采用成熟的细胞内感染实验[7]，测定了体外培养的3T3成纤维细胞的感染情况。将上述结果与采用赫斯特荧光染料（Hoechst）与5-乙炔基-2'-脱氧尿苷（EdU）联合检测法测得的3T3细胞内克氏锥虫（Trypanosoma cruzi）增殖情况进行对比。2) 针对克氏锥虫，泊沙康唑（POSA）所展现的最大抗虫活性（Emax）为75%，因此其半数抑制浓度（IC50）的计算基于次优效应水平。3) 显著性pmax值。其中NR表示未增殖（not replicating），Cmpd为化合物（compound），CF为富马酸氯马斯汀（clemastine fumarate），POSA为泊沙康唑（posaconazole），NFX为硝呋替莫（nifurtimox），BZ为苄硝唑（benznidazole）。