SNP data from OX1 human fibroblasts and induced Pluripotent Stem cells

human induced Pluripotent Stem cells (hiPSc) and their differentiated progeny have great potential for modelling disease. To realise this potential, robust protocols need to be developed for deriving authentic differentiated cell lineages and these lineages need to be rigorously characterised. We have generated hiPSc using retrovirus-mediated delivery of reprogramming factors, and have used them for assessing the efficiency of a defined protocol for differentiation to monocytes and macrophages. hiPSc lines have been screened using SNP array to assess chromosomal stability, and validation of the pluripotency of the hiPSc lines is provided by Pluritest assessment of transcriptome datasets. van Wilgenburg B, Browne C and Cowley SA, submitted for publication human iPSc lines were derived from normal human dermal fibroblasts.

人类诱导多能干细胞（human induced Pluripotent Stem cells，hiPSc）及其分化子代细胞在疾病建模领域拥有巨大应用潜力。为充分释放其应用价值，亟需建立稳健可靠的实验方案以获取纯正的分化细胞谱系，并对这些谱系开展严谨的表征分析。本研究通过逆转录病毒介导的重编程因子递送技术构建了hiPSc细胞系，并利用该细胞系评估了定向分化为单核细胞与巨噬细胞的标准化方案的效率。我们采用单核苷酸多态性芯片（SNP array）对hiPSc细胞系进行筛查，以评估其染色体稳定性；同时通过多能性检测平台（Pluritest）分析转录组数据集，完成了hiPSc细胞系多能性的验证。van Wilgenburg B、Browne C与Cowley SA已将相关研究成果投稿待发表，其构建的hiPSc细胞系源自正常人类真皮成纤维细胞。