Data for the "Discovery of Dehydroamino Acid Residues in the Capsid and Matrix Structural Proteins of HIV-1"

Bottom-up mass spectrometry-based proteomic analysis (trypsin) was performed on four biological replicates of HIV-1 virions. These virions were isolated from HEK293T cells transfected with a HIV-1 proviral plasmid derived from the pNL4-3 molecular clone, rendered biosafe due to inactivating point mutations in both the env and vpr reading frames. There are 8 total spectra, 4 are from unlabeled aliquots of sample, and 4 are from aliquots of sample treated with glutathione to label dehydroamino acids (Spectra can be accessed on MassIVE (MSV000088220). All data was analyzed using MetaMorpheus version 0.0.319 (https://github.com/smith-chem-wisc/MetaMorpheus). Provided here are the results of this analysis.

本研究对四份生物学重复的HIV-1病毒颗粒开展了基于胰蛋白酶酶解的自下而上质谱蛋白质组学分析。该病毒颗粒从转染了源自pNL4-3分子克隆的HIV-1前病毒质粒的HEK293T细胞中分离得到；此质粒因在env和vpr读码框中引入了失活点突变而具备生物安全性。本次实验共获得8张质谱图谱，其中4张来自未标记的样品等分试样，另外4张来自经谷胱甘肽处理以标记脱氢氨基酸的样品等分试样（质谱图谱可在MassIVE数据库（MSV000088220）中获取）。所有数据均使用MetaMorpheus 0.0.319版本进行分析（https://github.com/smith-chem-wisc/MetaMorpheus）。本数据集提供了本次分析的全部结果。