H3K36me3.BG3 ChIP-chip

modENCODE_submission_301 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determe the locations of 125 chromosomal proteins and histone modifications across the Drosophila melanogaster genome. The proteins and modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: CHIP-chip EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Cell Line: ML-DmBG3-c2; Tissue: CNS-derived cell-line; Developmental Stage: third instar larval stage; Genotype: y v f mal; Sex: Unknown; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Cell Line ML-DmBG3-c2; Antibody H3K36me3

modENCODE_submission_301 本提交源自Gary Karpen主持的模式生物DNA元件百科全书（modENCODE）项目。如需查阅完整modENCODE项目列表，请访问：http://www.genome.gov/26524648。
项目目标：本研究旨在确定黑腹果蝇（Drosophila melanogaster）全基因组范围内125种染色体蛋白质与组蛋白修饰的定位位点。本次研究涉及的蛋白质及修饰参与DNA复制、基因表达、基因沉默与遗传等核心染色体生物学功能。我们将采用基因组平铺芯片开展染色质免疫沉淀（Chromatin ImmunoPrecipitation, ChIP）实验。
实验初期将利用三种细胞系与两个胚胎阶段的染色质完成定位检测，后续将针对部分蛋白质，将分析范围拓展至另外四种动物组织/发育阶段。
数据使用条款与规范请参阅以下链接：http://www.genome.gov/27528022 及 http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。
关键词：ChIP-chip
实验类型：ChIP-chip
生物来源：细胞系：ML-DmBG3-c2；组织：中枢神经系统来源细胞系；发育阶段：三龄幼虫期；基因型：y v f mal；性别：未知；生物学重复数：4；实验因素：细胞系ML-DmBG3-c2；抗体：H3K36me3