Butyrophilin 3A1 binds phosphorylated antigens and stimulates human TCR γδ cells.. Homo sapiens

We used genetic approaches to identify butyrophilin 3A1 (BTN3A1) as the molecule that binds and presents phosphorylated antigens to Vγ9Vδ2 TCR–expressing cells. BTN3A1 represents a novel antigen–presenting molecule for the activation of Vγ9Vδ2 TCR–expressing cells which play important roles in human tumor immunity and host defense. Overall design: 3 replicates of 4 cell lines (12 samples total) were analysed. Data was RMA normalized, and genes that expressed above the negative control in all 4 cell lines from chromosome 6 considered for further analysis

本研究通过遗传学方法鉴定出嗜乳脂蛋白3A1（BTN3A1），该分子可结合并呈递磷酸化抗原至表达Vγ9Vδ2 T细胞受体（T cell receptor, TCR）的细胞。嗜乳脂蛋白3A1是一类新型抗原呈递分子，能够激活表达Vγ9Vδ2 TCR的细胞；此类细胞在人类肿瘤免疫与宿主防御过程中发挥关键作用。
实验设计：本研究对4种细胞系的3次生物学重复样本（总计12个样本）进行了分析。
数据经RMA（Robust Multi-array Average，稳健多数组平均）归一化处理后，本研究对来自6号染色体、且在全部4种细胞系中表达量均高于阴性对照的基因开展了后续分析。