β-catenin regulates FOXP2 transcriptional activity via multiple binding sites

We observed different gene expression by overexpressing the transcription factor FOXP2 in U2OS cells compared to mock-transfected cells. Additionally, we found β-catenin as co-activator binding directly to FOXP2. Activation of Wnt signaling was changing the transcriptional activity of FOXP2. Other functional and regulatory elements were found within FOXP2 and were confirmed by RNA-Seq. Determining gene expression differences by overexpressing an transcription factor (wildtype and mutant) in human osteosarcoma cells and additional activation of an Signaling pathway linked to the function of this transcription factor

本研究在U2OS细胞中过表达转录因子FOXP2，与空白转染（mock-transfected）的对照组细胞相比，观测到基因表达存在显著差异。此外，本研究发现β-连环蛋白（β-catenin）可作为共激活因子直接结合FOXP2；Wnt信号通路（Wnt signaling）的激活能够调控FOXP2的转录活性。另外，在FOXP2基因内部还鉴定出其他功能与调控元件，并通过RNA测序（RNA-Seq）得到了验证。本数据集通过在人骨肉瘤细胞中过表达该转录因子的野生型与突变体，并额外激活与该转录因子功能相关的信号通路，以探究对应的基因表达差异。