IL-17RA-signaling modulates CD8+ T cell survival, differentiation and exhaustion during Trypanosoma cruzi infection. IL-17RA-signaling modulates CD8+ T cell survival, differentiation and exhaustion during Trypanosoma cruzi infection

We used microarrays to compare gene expression profile of spleen CD8 T cells from IL-17RA KO and WT mice at different time-point after T. cruzi infection. Overall design: 9 WT mouse samples (3 samples at day 0 post infection -pi-, 3 samples at day 14pi and 3 samples at day 22pi); 9 IL-17RA KO mouse samples(3 samples at day 0 pi, 3 samples at day 14pi and 3 samples at day 22pi).

本研究利用基因芯片（microarrays）技术，比较克氏锥虫（Trypanosoma cruzi）感染后不同时间点，野生型（Wild Type, WT）与白细胞介素17受体A敲除（IL-17RA KO）小鼠的脾脏CD8阳性T细胞基因表达谱。整体实验设计：共包含9份野生型小鼠样本，其中感染后（post infection, pi）第0天、第14天、第22天样本各3份；另有9份白细胞介素17受体A敲除小鼠样本，在上述三个时间点各取3份。